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An automated HPLC method for the determination of aminoglycosides in serum using precolumn sample cleanup and derivatization
Summary
Type:
HPLC
Application #:
103400
Detection:
Conditions:
Fig. 3: eluent: 80% methanol - 0.1M aqueous Na acetate pH 7.4, flow rate 1ml/min
Fig. 5: eluent: 74% methanol - 0.25M aqueous Na acetate pH 7.4, flow rate 1ml/min
Authors:
Essers, L.
Source:
J. Chromatogr. 305 (1984) 345–352
Details
Legend: Fig. 3. Separation of gentamicin derivatives from blank peaks: A) short cation exchange column (arrow indicates badly resolved C1A and C2 compounds), B) reversed phase column, C) combination of both
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